Over the last one decade plant biotechnology has gained importance in the agricultural community. During this time period, plant tissue culture has traveled from the small laboratories and reached the mainstream. Plant tissue culture refers to the collection of ways which are used for growing large number of plant cells in a closely controlled environment. This technique works well as each cell has the genetic information and cellular machinery required for generating an entire organism and this technique could be put to use for producing a large number of plants which are genetically identical to a parent as well as to one another. The culture of tissue, plant cells and organs under aseptic conditions is undertaken in plant tissue culture techniques. An important role is played by these techniques in the production of horticultural, agricultural, ornamental plants and in manipulating plants for improved agronomic performance.